Abstract
A monoclonal antibody specific for DNA.multidot.RNA duplexes, particularly DNA.multidot.RNA heteropolymer duplexes, characterized by having cross-reactivity for binding to single- or double-stranded DNA or RNA as measured by competitive immunoassay of less than about 1:1000, and preferably less than 1:10,000, and an affinity for DNA.multidot.RNA heteropolymer duplexes greater than 10.sup.9 L/mole. The monoclonal antibody is prepared by conventional somatic cell hybridization techniques wherein the host animal is preferably immunized with an immunogen comprising a random DNA.multidot.RNA heteropolymer. The antibody, particularly in a labeled form, is useful in the specific detection of DNA.multidot.RNA duplexes in a test medium such as a nucleic acid hybridization assay mixture.
Filing date: Aug 26, 1985
Issue date: May 23, 1989
Inventor: Robert J. Carrico
Assignee: Miles Inc.
Primary Examiner: Jeremy M. Jay
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What is claimed is:
1. The monoclonal antibody secreted by hybridoma cell line ATCC HB 8730.
2. Hybridoma cell line ATCC HB 8730.