Abstract
A novel nucleic acid, arabinonucleic acid, is provided as a probe in nucleic acid assays. The arabinose moiety of the probe can be detected with anti-arabinose antibody-label conjugates.
Filing date: Dec 23, 1985
Issue date: Jul 26, 1988
Inventor: Randy M. McCormick
Assignee: E. I. Du Pont de Nemours and Company
Primary Examiner: Robert Benson
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What is claimed is:
1. A probe for the detection of a preselected nucleic acid sequence comprising single stranded arabinonucleic acid consisting essentially of arabinonucleotides having 3' and 5' internucleotide linkages with a base linked at the 1' position, said base being selected from the group consisting essentially of adenine, guanine, cytosine, thymine and uracil, said bases having a sequence complementary to said preselected nucleic acid sequence.
2. A method for identifying a preselected nucleic acid sequence in a sample comprising the steps of:
- (a) rendering the nucleic acid in said sample single-stranded;
- (b) immobilizing the single-stranded nucleic acids onto a support;
- (c) contacting said immobilized single-stranded nucleic acids with a single-stranded arabinonucleic acid consisting essentially of arabinonucleotides having 3' and 5' internucleotide linkages with a base linked at the 1' position, said base being selected from the group consisting essentially of adenine, guanine, cytosine, thymine and uracil, said bases having a sequence complementary to said preselected nucleic acid sequence, under conditions that allow a hybridization reaction to occur;
- (d) washing said support to remove arabinonucleic acid not incorporated into the hybrid formed on the support; and
- (e) determining the presence of arabinonucleic acid in the hybrid formed on the support by contacting it with an anti-arabinose antibody-label conjugate and detecting said label.
3. The method of claim 2 wherein the label is an enzyme.