Abstract
The invention relates to a multifunctional enzyme that can be derived from crustaceans or fish. The enzyme has at least one of a chymotrypsin, trypsin, elastase, collagenase and exo peptidase activity, and a molecular weight between about 20 kd and about 40 kd as determined by SDS PAGE. Preferably, the multifunctional enzyme has substantial anti cell-cell adhesion activity. Preferably, the multifunctional enzyme has substantial homology with the krill multifunctional enzyme. These enzymes are useful for treating viral infections such as herpes outbreaks, fungal, bacterial or parasitic infections, including the primary and secondary infections of leprosy, colitis, ulcers, hemorrhoids, corneal scarring, dental plaque, acne, cystic fibrosis, blood clots, wounds, immune disorders including autoimmune disease and cancer. Additionally, the invention relates to a method of purifying the multifunctional enzyme, and to a preparation of essentially purified multifunctional enzyme.
Filing date: Feb 8, 1996
Issue date: Sep 28, 1999
Inventors: Johan R. de Faire, Richard L. Franklin, John Kay, Ragnvald Lindblom
Assignee: Phairson Medical Inc.
Primary Examiner: Jay Williams
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What is claimed is:
1. A method of treating acne or eczema comprising topically administering an acne or eczema treating effective amount of a krill-derived multifunctional enzyme, wherein the multifunctional enzyme has at least two of a chymotrypsin, trypsin, collagenase, elastase or exo peptidase activity, a molecular weight between about 26 kd and about 32 kd as determined by SDS PAGE, and an N-terminal sequence comprising:
- I-V-G-G-X-E-V-T-P-H-A-Y-P-W-Q-V-G-L-F-I-D-D-M-Y-F (SEQ ID NO:20)
- wherein X is any amino acid.
2. The method of claim 1, wherein the enzyme has endo and exopeptidase activities.
3. The method of claim 1, wherein the enzyme has at least three of said proteolytic activities.
4. The method of claim 1, wherein the enzyme has at least four of said proteolytic activities.
5. The method of claim 1, wherein the enzyme has all of said proteolytic activities.
6. The method of claim 1, wherein the enzyme removes or inactivates at least one cell surface receptor selected from the group consisting of ICAM-1 (i.e., CD 54), ICAM-2, VCAM-1, CD4, CD8, CD28, CD29D, CD31, CD44, CD 49, CD62L, CD102 and the asialo GM1 ceramide.
7. The method of claim 6, wherein the enzyme removes or inactivates at least one cell surface receptor selected from the group consisting of ICAM-1, CD62L, CD4 and CD8.