Method of recollagenation

Abstract

A surgical method for implanting cadaver collagen for the restoration of lesions caused by the loss of collagen. The skin is perforated and a pocket is created under the skin. Human cadaver collagen is then introduced into the pocket and the skin perforation is closed. Over the next several months, the cadaver collagen is replaced by endogenous collagen.

Patent number: 5397352
Filing date: Aug 27, 1993
Issue date: Mar 14, 1995
Inventor: Steven Burres
Primary Examiner: Rob Clarke


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What is claimed is:

1. A method of recollagenation comprising:

a. identifying a skin depression for elevation;

b. perforating the skin with a needle adjacent to the said skin depression;

c. creating a pocket underneath the skin surface over said skin depression by swiveling said needle through the dermis;

d. cutting at least one strip or chip of freeze dried banked human fascia lata to be placed in said pocket;

e. soaking said strip or chip of fascia lata in a supersaturated sugar solution;

f. inserting said strip or chip of fascia lata through said skin surface above said pocket; and

g. closing said skin perforation.

2. The method of claim 1 wherein said skin depression is a scar.

3. The method of claim 2 wherein said scar is an acne scar.

4. The method of claim 2 wherein said scar is a chicken pox scar.

5. The method of claim 1 wherein said skin depression is a wrinkle.

6. The method of claim 1 wherein said skin depression is a fatty depression commonly referred to as cellulite.

7. The method of claim 1 wherein said strip or chip of banked human fascia lata is cut to the same size of said pocket.

8. The method of claim 1 wherein said supersaturated sugar solution is a supersaturated glucose solution.

Treated tissue for implantation and methods of preparation

Abstract

This disclosure includes a method for generating a functional hybrid bioprosthesis. Tissue formed naturally of interstitial collagens is treated to kill native cells and remove potentially immunologically active soluble molecules. Then it may be treated sequentially with extracellular matrix adhesion factor, extracellular matrix glycosaminoglycan, and growth factor appropriate to the cell type required to function within the matrix, and incubating the transplant tissue matrix with cells that are either allogeneic or autologous for the recipient thereby imparting to the matrix the characteristics of the cell type and tissue selected. Tissues with a variety of functional bioactivities can thus be formed in vitro prior to graft transplantation or implantation which will exhibit reduced or no stimulation of an immunological response in the recipient.

Patent number: 5632778
Filing date: Jun 5, 1995
Issue date: May 27, 1997
Inventor: Steven Goldstein
Assignee: Cryolife, Inc


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What is claimed is:

1. A process for generating a substantially non-immunogenic tissue matrix suitable for subsequent processing into an implant tissue comprising:

A. eliminating native cells by treating a tissue with components selected from the group consisting enzymes and nucleases effective to inhibit subsequent native cell growth in the treated tissue and effective to limit generation of new immunological sites in the tissue thus producing a tissue matrix;

B. treating the tissue matrix with cellular adhesion factor to promote subsequent attachment of cultured allogeneic or autologous cells to the surfaces of the tissue matrix; and

C. repopulating the tissue matrix throughout the matrix with cultured allogeneic or autologous cells.

2. The process of claim 1 wherein, during step A, the tissue is treated with a low ionic strength solution of nucleases effective to decellularize the tissue and provide a tissue matrix of limited immunogenicity.

3. The process of claim 2 wherein the low ionic strength solution of nucleases includes at least one member, selected from the group consisting of RNAase A, DNAase I, EcoR I and Hind III.

4. The process of claim 2 wherein the nucleases employed are ribonuclease A and deoxyribonuclease I.

5. The process of claim 1 further comprising cryopreserving the tissue matrix after step A and prior to further processing.

6. The process of claim 2 further comprising cyropreserving the tissue matrix after step A and prior to further processing.

7. The process of claim 5 further comprising sterilizing the tissue or tissue matrix or both.

8. The process of claim 1 wherein the process further comprises obtaining natural mammalian tissue, cryopreserving the tissue, removing the tissue from cryopreservation and then treating the tissue in accordance with claim 1.

9. The process of claim 8 wherein the tissue is of porcine heart valve origin.

10. The process of claim 9 wherein the heart valve is a pulmonary or an aortic heart valve.

11. The process of claim 1 wherein step A further comprises treatment with a hypotonic aqueous solution.

12. The process of claim 11 herein said hypotonic aqueous solution is a buffered aqueous solution effective to cause cell lysis.

13. The process of claim 12 wherein the tissue is porcine heart valve tissue.

14. The process of claim 13 wherein the heart valve is a pulmonary or an aortic heart valve.

15. The process of claim 14 further comprising the step of cryopreserving the porcine heart valve tissue matrix produced by step A.

Breast prosthesis with biologically absorbable outer container

Abstract

A newly developed breast prosthesis overcomes the tightness and contracture of the fibrous capsule which forms around the existing prosthesis. The unique construction of the new prosthesis causes the capsule to form at a predetermined, controlled distance from the surface thereof. This prosthesis is constructed with a first phase or outer temporary component and a second phase or inner permanent component. The inner component is a container or sac of a flexible, non-absorbable material filled with a fluid or gel filler material. The temporary outer component is an outer container or cover of a material which is absorbable under the conditions of use, and an inert filler material, preferably an absorbable, biologically acceptable liquid, e.g. saline solution, filling the space between the inner and outer components. The inner component is preferably of silicone rubber film and is filled with a silicone gel. The outer portion is in the form of a sheet, film or coating of a material...

Patent number: 4298998
Filing date: Dec 8, 1980
Issue date: Nov 10, 1981
Inventor: Sadeque S. Naficy


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What is claimed is:

1. A breast prosthesis comprising an inner core of biologically compatible, non-absorbable material, and

biologically absorbable means surrounding said core for effecting capsule formation at a selected and controlled distance from said core without contractive pressure thereon after surgical implantation.

2. A breast prosthesis according to claim 1 in which

said means comprises a flexible wall covering or container surrounding said inner core,

said biologically absorbable flexible wall covering or container at least at the time of surgical implantation having a biologically compatible liquid in the space between said flexible wall covering or container and said core.

3. A breast prosthesis comprising

an inner coherent core of biologically compatible, non-absorbable material and

an outer covering of absorbable material having an outer surface spaced from the surface of said core.

4. A breast prosthesis according to claim 2 in which

said absorbable material is a material selected from the group consisting of materials which are absorbed by hydrolysis, materials which are absorbed by phagocytosis, materials which are absorbed by enzymatic decomposition and materials which are absorbed by chemical or biological reactions.

5. A breast prosthesis according to claim 3 in which

said absorbable material is a material which is formed in situ by reaction of an initially non-absorbable material with added chemicals or enzymes.

6. A breast prosthesis according to claim 5 in which

said chemicals or enzymes are added to said initially non-absorbable material at the time of manufacture.

7. A breast prosthesis according to claim 5 in which

said chemicals or enzymes are added to said initially non-absorbable material in admixture with fluid introduced inside said outer covering.

8. A breast prosthesis according to claim 7 in which

said chemicals or enzymes are added to said fluid after surgical implantation of said prosthesis.

9. A breast prosthesis according to claim 5 in which

said chemicals or enzymes are added to said initially non-absorbable material at the time of surgical implantation of said prosthesis.

10. A breast prosthesis according to claim 3 in which

said absorbable material is a material which is formed in situ by physical treatment of an initially non-absorbable material to render the same absorbable.

11. A breast prosthesis according to claim 3 in which

said outer covering of absorbable material comprises

an outer container of a flexible absorbable material spaced from said core, and

an inert filler material filling the space between said outer container and said core.

12. A breast prosthesis according to claim 11 in which

said outer container comprises a film of a natural or synthetic or semisynthetic absorbable material.

13. A breast prosthesis according to claim 11 in which

said outer container comprises a film of a material selected from the group consisting of catgut, chromic gut, reconstituted animal collagen, reconstituted vegetable protein, absorbable carbohydrate polymers, and absorbable synthetic or semisynthetic polymers.

14. A breast prosthesis according to claim 13 in which

said film is an absorbable polyester of a dibasic acid.

15. A breast prosthesis according to claim 14 in which

said film is an absorbable condensation polymer of a hydroxycarboxylic acid.

16. A breast prosthesis according to claim 15 in which

said polymer is polyglycolic acid or polyglactin 910.

17. A breast prosthesis according to claim 11 in which

said outer container comprises a film of an absorbable polymer of cellulose or amylose or derivatives thereof.

18. A breast prosthesis according to claim 11 in which

said inert filler material is a biologically compatible solution.

19. A breast prosthesis according to claim 11 in which

said inner core comprises an inner container of a biologically compatible non-absorbable film enclosing a quantity of fluid material.

20. A breast prosthesis according to claim 19 in which

said fluid material in said inner container is a biologically compatible liquid.

21. A breast prosthesis according to claim 19 in which

said fluid material in said inner container is a gel material.

22. A breast prosthesis according to claim 19 in which

said inner core comprises a container of silicone rubber film enclosing a quantity of silicone gel.

23. A breast prosthesis according to claim 11 in which

said inner core comprises a non-absorbable sponge of a biologically compatible material.

24. A breast prosthesis according to claim 11 in which

said inner core comprises a sponge enclosed in a biologically compatible, non-absorbable film.

25. A breast prosthesis according to claim 3 in which

said outer covering comprises an absorbable sponge.

26. A breast prosthesis according to claim 3 in which

said inner core comprises a double lumen prosthesis.